Histopathological Detection of Mycobacterium Tuberculosis and Mycobacterium Leprae using a Modified Acid-Fast Technique

Authors

Keywords:

acid-fast bacilli, Mycobacterium tuberculosis, Mycobacterium leprae, tuberculosis, leprosy, Ziehl-Neelsen, Fite Faraco

Abstract

Introduction. Mycobacterium tuberculosis and Mycobacterium leprae are acid-fast organisms with lipid-rich cell walls that resist decolorization with acidified alcohol after application of a dye with heat. The Ziehl-Neelsen and Fite Faraco staining technique, which are diagnostic tools for identification of acid-fast bacilli (AFB) found in histopathologic samples, are based on this principle. A modification of the Ziehl-Neelsen technique is described as an alternative rapid and reliable method of diagnosis for prompt detection and treatment.

Methodology. One hundred and seven (107) archived tissue specimens from autopsy and dermatology cases interpreted as positive for M. tuberculosis and M. leprae were stained using the proposed modified acid-fast (MAF) technique compared with Fite Faraco (FF) staining method as reference standard. Each specimen was read by two independent evaluators.

Results. The degree of diagnostic agreement of the MAF with FF was calculated. For autopsy (n=16) and dermatology (n=91) samples, the Cohen’s kappas are 0.765 (substantial) and 0.397 (fair), respectively. Overall, the Cohen’s kappa is 0.458 (moderate).

Conclusion. The proposed modified Acid-Fast staining method may be considered as an alternative to the conventional Ziehl-Neelsen method and the Fite Faraco method in identifying positive acid-fast bacilli in tissue samples taken from clinical cases of M. tuberculosis and M. leprae.

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Author Biographies

Estatera Cabic, Research Institute for Tropical Medicine-Department of Health

Medical Technologist III

Alpha Grace Cabic, Research Institute for Tropical Medicine-Department of Health

Medical Specialist II (PT)

Sheila Marie Esposo, Research Institute for Tropical Medicine-Department of Health

Medical Specialist II (PT)

Gloria June Quinones, Research Institute for Tropical Medicine-Department of Health

Science Research Specialist I

Arnel Guia, Research Institute for Tropical Medicine-Department of Health

Science Research Specialist II

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Published

04/17/2018

How to Cite

Cabic, E., Cabic, A. G., Esposo, S. M., Dizon, F., Quinones, G. J., & Guia, A. (2018). Histopathological Detection of Mycobacterium Tuberculosis and Mycobacterium Leprae using a Modified Acid-Fast Technique. PJP, 3(1), 29. Retrieved from https://philippinejournalofpathology.org/index.php/PJP/article/view/85

Issue

Section

Diagnostic Perspectives

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