Alocilja Magnetic Nanoparticles Efficiently Capture Escherichia coli O157:H7 Isolates

Keywords: Alocilja magnetic nanoparticles, E. coli O157, H7, TEM images, cell capture

Abstract

Escherichia coli O157:H7 (EcO157) is a notorious foodborne pathogen known to cause bloody diarrhea and can even lead to death. Current detection methods, though highly sensitive, are lengthy and labor-intensive thus an alternative that is simple, rapid, low-cost and equally sensitive is necessary. Hence, an enabling method is the use of functionalized Alocilja magnetic nanoparticles (AMN), known to have high surface reactivity and can easily capture target biomolecules without the use of antibodies, such as microbial cells, in crude samples by means of a magnet. AMN, patented after its inventor Dr. Evangelyn Alocilja, is composed of iron oxide/glycan core/shell structure with an average size of 180-450 nm and with superparamagnetic properties. AMN has been reported to capture Salmonella enterica, Bacillus cereus and Mycobacterium smegmatis without the use of antibodies or peptides.

Author Biographies

Dodge Lim, Research Institute for Tropical Medicine-Department of Health
Head, Policy and Research Unit, National TB Reference Laboratory
Rovi Gem Villame, University of the Philippines MIndanao
Assistant Professor I, Department of Food Science and Chemistry, College of Science and Mathematics
Gloria June Quiñones, Research Institute for Tropical Medicine-Department of Health
Science Research Specialist I, Electron Microscopy Laboratory
Rebecca Notorio, Research Institute for Tropical Medicine-Department of Health
Science Research Specialist II, Electron Microscopy Laboratory
Lilia Fernando, University of the Philippines Los Baños
Head, Nano-Biotechnology Innovations and Translational Research Laboratory, National Institute of Molecular Biology and Biotechnology (BIOTECH)
Evangelyn Alocilja, Michigan State University
Professor/Program Director, Alocilja Nano-Biosensor Lab, Biosystems and Agricultural Engineering, Michigan State University

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Published
2017-11-18
How to Cite
Lim, D., Villame, R. G., Quiñones, G. J., de Vera, D., Notorio, R., Fernando, L., & Alocilja, E. (2017). Alocilja Magnetic Nanoparticles Efficiently Capture Escherichia coli O157:H7 Isolates. Philippine Journal of Pathology, 2(2), 47. Retrieved from https://philippinejournalofpathology.org/index.php/PJP/article/view/77
Section
Diagnostic Perspectives