Alocilja Magnetic Nanoparticles Efficiently Capture Escherichia coli O157:H7 Isolates

Dodge Lim, Rovi Gem Villame, Gloria June Quiñones, Dave de Vera, Rebecca Notorio, Lilia Fernando, Evangelyn Alocilja

Abstract

Escherichia coli O157:H7 (EcO157) is a notorious foodborne pathogen known to cause bloody diarrhea and can even lead to death. Current detection methods, though highly sensitive, are lengthy and labor-intensive thus an alternative that is simple, rapid, low-cost and equally sensitive is necessary. Hence, an enabling method is the use of functionalized Alocilja magnetic nanoparticles (AMN), known to have high surface reactivity and can easily capture target biomolecules without the use of antibodies, such as microbial cells, in crude samples by means of a magnet. AMN, patented after its inventor Dr. Evangelyn Alocilja, is composed of iron oxide/glycan core/shell structure with an average size of 180-450 nm and with superparamagnetic properties. AMN has been reported to capture Salmonella enterica, Bacillus cereus and Mycobacterium smegmatis without the use of antibodies or peptides.

Keywords

Alocilja magnetic nanoparticles, E. coli O157:H7, TEM images, cell capture

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References

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