Validation using Nucleic Acid Testing (NAT) on Blood Donor Samples Non-Reactive to Transfusion-Transmissible Viruses by Immunoassay (EIA/ChLIA)

Authors

  • Rhoda Yu Research Institute for Tropical Medicine
  • Iza Mae Chamen Research Institute for Tropical Medicine http://orcid.org/0000-0002-8088-0292
  • Kenneth Aristotle Punzalan Research Institute for Tropical Medicine http://orcid.org/0000-0002-0564-2331
  • Benjamin De Vera III Research Institute for Tropical Medicine
  • Elizabeth Arcellana-Nuqui The Medical City

Keywords:

blood transfusions, human immunodeficiency virus, hepatitis, transfusion transmissible infections, nucleic acid test

Abstract

Transfusion-transmissible infections (HIV, HBV and HCV) remain a threat to public health specifically in assuring safe transfusion practices. This study aims to determine the ability of a blood service facility to accurately detect HIV, Hepatitis B and C and assess the need to include nucleic acid testing as part of the routine screening algorithm. Of the 3,233 samples from participants with screened sero-negative blood units, testing for HIV and Hepatitis C showed no discrepancies with EIA and NAT in all samples. Testing for Hepatitis B showed 12 (4.00%) samples which are reactive in both EIA and NAT, 3 (0.09%) samples were reactive with EIA only and 48 (1.48%) were detected for the presence of Hepatitis B Virus via NAT.

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Author Biography

Kenneth Aristotle Punzalan, Research Institute for Tropical Medicine

Medical Specialist IV; Head-Department of Virology

References

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Published

12/19/2016

How to Cite

Yu, R., Chamen, I. M., Punzalan, K. A., De Vera III, B., & Arcellana-Nuqui, E. (2016). Validation using Nucleic Acid Testing (NAT) on Blood Donor Samples Non-Reactive to Transfusion-Transmissible Viruses by Immunoassay (EIA/ChLIA). PJP, 2(1), 39. Retrieved from https://philippinejournalofpathology.org/index.php/PJP/article/view/49

Issue

Section

Brief Communications