The Effect of Number of Tests, Hemoglobin Level and Working Temperature on the Specific Gravity of 100 ml Copper Sulphate Solution in Hemoglobin Screening

Authors

  • Francis Dematera Jose R. Reyes Memorial Medical Center FEU-NRMF Institute of Medicine Quezon City General Hospital Unihealth-Parañaque Hospital and Medical Center
  • Linda Tamesis School of Medicine, Institute of Medicine, Far Eastern University-Nicanor Reyes Medical Foundation

Keywords:

blood, hemoglobin, copper sulphate

Abstract

Background. The copper sulphate method, being economical, was used for hemoglobin screening in blood donation. Various references cite different number of tests that can be performed in a 100 mL copper sulphate solution.

Objective.The study was conducted to determine the effect of number of tests, hemoglobin level and working temperature on the specific gravity of 100 ml copper sulphate solution.

Methodology. Three groups of samples of known hemoglobin levels (<12 g/dl, 12-14 g/dl, and >14 g/dl) were tested using a 100 ml copper sulphate solution with specific gravity 1.053 at room temperature and at temperature of 29-30°C. Specific gravity of the solution was measured after every 5 tests for a total of 50 tests per experiment.

Result.There was no change in the specific gravity of copper sulphate solution used in 50 tests. There was no difference in the measured specific gravity across all experiments.

Conclusion. A 100 mL copper sulphate solution can be used for 50 tests using samples of various hemoglobin levels, at room temperature and at a higher temperature.

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Author Biographies

Francis Dematera, Jose R. Reyes Memorial Medical Center FEU-NRMF Institute of Medicine Quezon City General Hospital Unihealth-Parañaque Hospital and Medical Center

Medical Specialist I, Department of Pathology, Jose R. Reyes Memorial Medical Center

Assistant Professor, Department of Pathology, FEU-NRMF

Training Officer (CP), Department of Pathology, Quezon City General Hospital

Chief Pathologist, Department of Pathology, Unihealth-Parañaque Hospital and Medical Center

Linda Tamesis, School of Medicine, Institute of Medicine, Far Eastern University-Nicanor Reyes Medical Foundation

Dean

References

1. Tondon R, Verma A, Pandey P, and Chaudhary R. Quality evaluation of four hemoglobin screening methods in a blood donor setting along with their comparative cost analysis in an Indian scenario. Asian J Transfus Sci. 2009;3(2):66-9. https://doi.org/10.4103/0973-6247.53874.

2. Problems with QC of Copper Sulfate? E-Network Forum-California Blood Bank Society.

3. Guidelines for blood transfusion services in the United Kingdom, 7th ed., UK blood transfusion and tissue transplantation services, 2005. Available online: https://www.gov.uk/government/uploads/system/uploads/attachment_data/file/228828/0117033715.pdf.

4. Model standard operating procedures for blood transfusion service. World Health Organization, New Delhi, 2002. Available online: http://apps.searo.who.int/PDS_DOCS/B0235.pdf?ua=1.

5. Philips RA, Van Slyke DD, Hamilton PB, Dole VP, Emerson K, Archibald RM. Measurement of specific gravities of whole blood and plasma by standard copper sulfate solution. J Biol Chem. 1950;183:305-30. Available online: http://www.jbc.org/content/183/1/305.full.pdf+html.

6. Anemia detection methods in low-resource settings: a manual for health workers. Seattle, Washington, USA: PATH Publications, 1997. Available online: http://www.path.org/publications/files/TS_anemia_guide_health_workers.pdf.

7. Sawant RB, Bharucha ZS, Rajadhyaksha SB.Evaluation of hemoglobin of blood donors deferred by copper sulfate method for hemoglobin screening. Transfus Apher Sci. 2007;36(2):143-8. PMID: 17382593. https://doi.org/10.1016/j.transci.2006.11.001.

8. Brecher ME, American Association of Blood Banks. Technical Manual, 15th ed. Bethesda, Md.: American Association of Blood banks, 2005. Available online: http://www.eqas.ir/pdf/lib/AABB%20Technical%20Manual%2015TH.pdf.

9. Mannarino AD, Macpherson CR. Copper sulfate screening of blood donors: report of a donor passing the test with less than eight grams of hemoglobin. Transfusion:1963;3:398-400. PMID: 14077075.

10. McPherson RA, Pincus MR, Henry JB. Henry's clinical diagnosis and management by laboratory methods, 21st ed., Philadelphia: Saunders Elsevier, 2007. NLIM ID: 101289889 [Book].

Published

09/07/2017

How to Cite

Dematera, F., & Tamesis, L. (2017). The Effect of Number of Tests, Hemoglobin Level and Working Temperature on the Specific Gravity of 100 ml Copper Sulphate Solution in Hemoglobin Screening. PJP, 2(2), 6. Retrieved from https://philippinejournalofpathology.org/index.php/PJP/article/view/29

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Section

Original Articles