The Effect of Number of Tests, Hemoglobin Level and Working Temperature on the Specific Gravity of 100 ml Copper Sulphate Solution in Hemoglobin Screening

Authors

  • Francis Dematera Jose R. Reyes Memorial Medical Center FEU-NRMF Institute of Medicine Quezon City General Hospital Unihealth-Parañaque Hospital and Medical Center
  • Linda Tamesis School of Medicine, Institute of Medicine, Far Eastern University-Nicanor Reyes Medical Foundation

Keywords:

blood, hemoglobin, copper sulphate

Abstract

Background. The copper sulphate method, being economical, was used for hemoglobin screening in blood donation. Various references cite different number of tests that can be performed in a 100 mL copper sulphate solution.

Objective.The study was conducted to determine the effect of number of tests, hemoglobin level and working temperature on the specific gravity of 100 ml copper sulphate solution.

Methodology. Three groups of samples of known hemoglobin levels (<12 g/dl, 12-14 g/dl, and >14 g/dl) were tested using a 100 ml copper sulphate solution with specific gravity 1.053 at room temperature and at temperature of 29-30°C. Specific gravity of the solution was measured after every 5 tests for a total of 50 tests per experiment.

Result.There was no change in the specific gravity of copper sulphate solution used in 50 tests. There was no difference in the measured specific gravity across all experiments.

Conclusion. A 100 mL copper sulphate solution can be used for 50 tests using samples of various hemoglobin levels, at room temperature and at a higher temperature.

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Author Biographies

Francis Dematera, Jose R. Reyes Memorial Medical Center FEU-NRMF Institute of Medicine Quezon City General Hospital Unihealth-Parañaque Hospital and Medical Center

Medical Specialist I, Department of Pathology, Jose R. Reyes Memorial Medical Center

Assistant Professor, Department of Pathology, FEU-NRMF

Training Officer (CP), Department of Pathology, Quezon City General Hospital

Chief Pathologist, Department of Pathology, Unihealth-Parañaque Hospital and Medical Center

Linda Tamesis, School of Medicine, Institute of Medicine, Far Eastern University-Nicanor Reyes Medical Foundation

Dean

References

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Published

2017-09-07

How to Cite

Dematera, F., & Tamesis, L. (2017). The Effect of Number of Tests, Hemoglobin Level and Working Temperature on the Specific Gravity of 100 ml Copper Sulphate Solution in Hemoglobin Screening. PJP, 2(2), 6. Retrieved from https://philippinejournalofpathology.org/index.php/PJP/article/view/29

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Section

Original Articles